Double‐strand breaks arising by replication through a nick are repaired by cohesin‐dependent sister‐chromatid exchange
نویسندگان
چکیده
منابع مشابه
Double-strand breaks arising by replication through a nick are repaired by cohesin-dependent sister-chromatid exchange.
Molecular studies on double-strand break (DSB) repair in mitosis are usually performed with enzymatically induced DSBs, but spontaneous DSBs might arise because of replication failures, for example when replication encounters nicks. To study repair of replication-born DSBs, we defined a system in Saccharomyces cerevisiae for the induction of a site-specific single-strand break. We show that a 2...
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In haploid rad52 Saccharomyces cerevisiae strains unable to undergo homologous recombination, a chromosomal double-strand break (DSB) can be repaired by imprecise rejoining of the broken chromosome ends. We have used two different strategies to generate broken chromosomes: (i) a site-specific DSB generated at the MAT locus by HO endonuclease cutting or (ii) a random DSB generated by mechanical ...
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ژورنال
عنوان ژورنال: EMBO reports
سال: 2006
ISSN: 1469-221X,1469-3178
DOI: 10.1038/sj.embor.7400774